KROMATON is an innovating firm pioneer in Centrifugal Partition Chromatography (CPC), or Countercurrent Chromatography (CCC), a liquid/liquid preparative chromatography technique.
Kromaton – centrifugal partition chromatography
Since 1999, Kromaton has been developing a wide range of complete FCPC systems (Fast Centrifugal Partition Chromatography), for the extraction, separation and purification of natural, biological or synthetic molecules. It is considered today as the true and proven alternative to standard techniques such as HPLC or Flash Chromatography (no costly solid packing material, no sample loss and 100% recovery, no denaturation of fragile molecules, low solvent consumption, etc…). Its range of Centrifugal Partition Chromatography systems FCPC are designed and manufactured for research and development laboratories, pilot plants as well as industrial production plants in the chemical-pharmaceutical industries, cosmetics, biotechnologies or natural products field.
Centrifugal Partition Chromatography is also known as Hydrostatic Countercurrent Chromatography. This refers to the rotary movement of the column which rotates around one single rotation axis, contrary to Hydrodynamic Countercurrent Chromatography systems that have a planetary rotation movement around two rotating axes. Centrifugal Partition Chromatography is a liquid-liquid chromatography technique. The Kromaton Fast Centrifugal Partitioning Chromatograph thus functions based on the principles of liquid/liquid partitioning chromatography: two immiscible liquid phases are mixed together to form a two-phase system, and are then separated multiple times. The individual solutes are isolated based on the different partitioning coefficients of each compound in this two-phase system. One of the liquid phases of the two-phase system is used as a stationary liquid phase: it is fed into the column (the rotor) while the latter is spinning at moderate rotational speed. The stationary phase is retained inside the rotor by the centrifugal force generated. The second phase of the two-phase system is used as the mobile phase containing the solutes to be extracted. It is fed under pressure into the rotor and pumped through the stationary phase. Both phases are mixed together. It is at that time that the exchange of molecules between the two phases occurs. The separation of the solutes is achieved as a function of the specific partitioning coefficient (Kd) of each solute between the mobile and stationary phases. The mobile phase then decants at each cell outlet thus entering the next cell.
All Kromaton’s FCPC Centrifugal Partition Chromatography systems are designed and manufactured in-house, in its facilities located in Annonay (France).
More about Kromaton : http://kromaton.com